Now that I am well started, it is time to tell you a bit about my project. This summer, 410 patients, all affected by gliomas (i.e brain tumours), had their DNA extracted to characterise their mutational status. Therefore, my internship goal is to amplify genes of interest (named IDH1, IDH2 and Tert [2,3]) by PCR to allow Sanger sequencing and then investigate potential mutations. Ultimately, I will carry out statistical analyses to estimate the impact of each mutation on survival and to compare my results with the available bibliography. Feel free to ask me anything about this project! References:
[1] YouTube. (2017). Metallica & San Francisco Symphony Orchestra - Master Of Puppets. [online] Available at: https://www.youtube.com/watch?v=A39C6o6x-fU [Accessed 29 Mar. 2017]. [2] Labussière, M., Di Stefano, A., Gleize, V., Boisselier, B., Giry, M., Mangesius, S., Bruno, A., Paterra, R., Marie, Y., Rahimian, A., Finocchiaro, G., Houlston, R., Hoang-Xuan, K., Idbaih, A., Delattre, J., Mokhtari, K. and Sanson, M. (2014). TERT promoter mutations in gliomas, genetic associations and clinico-pathological correlations. British Journal of Cancer, 111(10), pp.2024-2032. [3] Boisselier, B., Gallego Perez-Larraya, J., Rossetto, M., Labussiere, M., Ciccarino, P., Marie, Y., Delattre, J. and Sanson, M. (2012). Detection of IDH1 mutation in the plasma of patients with glioma. Neurology, 79(16), pp.1693-1698. For a short-but-still-useful reminder of what a PCR is: YouTube. (2017). PCR - Polymerase Chain Reaction (IQOG-CSIC). [online] Available at: https://www.youtube.com/watch?v=iQsu3Kz9NYo [Accessed 29 Mar. 2017]. If you want to know more about Sanger sequencing, gold standard method to look for mutants: Thermofisher.com. (2017). Sanger Sequencing Method | Thermo Fisher Scientific. [online] Available at: https://www.thermofisher.com/fr/fr/home/life-science/sequencing/sanger-sequencing/sanger_sequencing_method.html# [Accessed 30 Mar. 2017].
7 Comments
Clara Haas
30/3/2017 04:50:47 pm
Hi Margaux!
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margaux
31/3/2017 12:36:54 am
Dear Clara,
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Margaux (yeah, me again...)
1/4/2017 11:26:20 am
I made a tiny istake: the primers doesn't matter for Tert (it may for IDH2, I will have a look at that) so the double peak is only due to either both cellular types or/and heterozygosity
Régine
31/3/2017 03:48:55 am
Hello Margaux,
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Margaux
1/4/2017 11:21:58 am
Hello Régine!
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Cécile
1/4/2017 10:49:31 am
Hi!
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Margaux
1/4/2017 11:29:58 am
Hey Cécile!
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