-Remember, my internship is about fungal strains that can dissolve plastic pollutants, such as Biphenol A, on waste-waters... Hello my friends ! In order to measure the degradation of plastic by fungi in a liquid media, we have to measure the pollutant concentration before and during the fungal enzymatic activity. In order to quantitate the three pollutants, we use a UPLC – Ultra Performant Liquid Chromatography. This machine permit to identify components in a liquid phase with a huge precision. On this article, I will give more explanation about this machine …. A pokemon evolution is a change in the shape of the pokemon, resulting a new pokemon that will be stronger than the previous one, more powerful, faster, more beautiful... As an example, Paras, the mushroom pokemon, evolve to Parasect at the level 24; Parasect is (relatively) better... For our chromatography technology, it's the same! UPLC (Ultra-Performant-Liquid-Chromatography) technology, released in 2004, is much more accurate to separate compounds in a mixture than HPLC (Hight-Performat-L-C), first designed in 1952. UPLC is a patented trademark from the Waters Corporation. Its a technology similar to the HPLC with advanced features. This is how the machine look like : The process cover by this machine is called UHPLC – but the trademark is commonly used to name the process, removing the H. This malapropism happen in other situations, such as “-Margaux, do you have a Kleenex?” or “-Margaux, could you give me your scotch?” instead of “-Margaux, do you have a tissue, and could you give me the [whisky] adhesive tape...” The chromatography separate components in a mixture, permit their identification and can quantitate and even isolate them. UPLC deliver data, visual spectrum and associated values for each peaks that can be analysed quickly by scientists, even with internship students. It is composed by several complementary features: Pumps and Solvent Reservoir, The Automatic Sampling, The Column, the PDA detector and the tubing.
UPLC bonus: a lower volume of solvent is need to inject the mobile phase: Where 30µL were previously needed, only 4.97 µL is used with the new technology. The binary solvent distribution is managed by the PLAST4 method: from the beginning until the fifth minute, the water solvent is injected, and hydrophilic compounds are first passing through the columns. The water percentage decrease hugely and Methanol is add from the fifth minutes during 30 seconds, and hydrophobic compounds start to go through the columns. In the spectrum, a huge number of peak appeared at this period. The Methanol is injected until the seventh minutes.
UPLC malus: lifetime of columns are usually shorter...
Fun fact: A shift in time of released compound happened during my experiment: the same compound was released belatedly than at the beginning. I have to know its an error from the machine or if it’s something else … I first wanted to draw a scheme but now I am too lazy ! Hope you have understand something useful :D Bibliography :
Wiki : https://fr.wikipedia.org/wiki/Chromatographie_en_phase_liquide_%C3%A0_haute_performance https://en.wikipedia.org/wiki/High-performance_liquid_chromatography https://fr.wikipedia.org/wiki/UPLC https://en.wikipedia.org/wiki/Van_Deemter_equation video : https://www.canal-u.tv/video/cerimes/chromatographie_liquide_a_haute_performance.9085 http://www.lachimie.fr/analytique/chromatographie/HPLC/HPLC.php http://www.waters.com/waters/fr_FR/UPLC---Ultra-Performance-Liquid-Chromatography-Beginner%27s-Guide/nav.htm?cid=134803622&locale=fr_FR http://www.waters.com/waters/fr_FR/Original-UPLC-UHPLC-system-with-sub-2-micron-particle-technology-for-separations/nav.htm?cid=514207&locale=fr_FR PWP : https://www.slideshare.net/manubhau/ultra-performance-liquid-chromatography-pptby-manoj-ingale https://www.slideshare.net/ShaimaaAhmadeen/1-14411077 http://www.americanlaboratory.com/913-Technical-Articles/1569-Effective-UPLC-Implementation/
3 Comments
Isabelle
12/4/2017 10:40:37 am
Hello Yuyu ! What is the limitation of this technologie ? All substances are compatible ? And how much of substance can be identify (maximal) ?
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Hortense
14/4/2017 02:45:05 am
Dear Yuyu,
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Margaux
20/4/2017 04:28:11 am
I'm flattered to be in your post :D
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